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This title presents state-of-the-art update on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. It provides state-of-the-art update on methods and protocols. It deals with the detection, isolation and characterization of macromolecules and their hosting organisms. It also deals with the challenges of very diverse environments.