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With enzymes being the most valued and common of drug targets, an understanding of their interactions with inhibitors is critical to successful drug discovery. Now in a second edition, this proven work clearly explains the biochemical data and experimental details underlying the science, arming medicinal chemists and pharmacologists with the tools they need to master the art of applied enzymology for drug discovery. With updated material throughout, two new chapters, and five new appendices, Evaluation of Enzyme Inhibitors in Drug Discovery, Second Edition remains the only book available on the topic.