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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM ) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.§Covers many areas of CLEM by the best international scientists in the field. §Includes a brief history and overview on CLEM methods