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This book is intended as a reference book for engineering students in Physics and Biological field.The present book, Biophysics-An Introduction gives a vibrant scientific field where scientists from many fields including math, chemistry, physics, engineering, pharmacology, and materials sciences, use their skills to explore and develop new tools for understanding how biology-all life-works. The Biophysics major also serves as an excellent pathway for students intending to pursue postgraduate study. 'Biophysics' implies physics applied to biology: is that what biophysics is? Yes, biophysics is the study of biological systems and biological processes using physics-based methods or based on physical principles. We are thankful to god & our parents. We also give great thanks to our institution for their full support to publish this book.