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This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. The key features include: adenylyl cyclase: determination of activity; preparation of materials useful in purification of components of hormonally responsive cyclase systems; purification guanine nucleotide-dependent regulatory proteins; purification and characterization of g proteins: labeling and quantitating of G proteins; reconstitution guanylyl cyclase: assay; preparation of materials used for study of the cyclase; purification; and, regulation.