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Proteomische Methoden

Charakterisierung von phosphorylierungsvermittelten Wechselwirkungen des Adapterproteins ADAP

Language GermanGerman
Book Paperback
Book Proteomische Methoden Sabine Lange
Libristo code: 06993712
Eine Vielzahl von Protein-Protein-Wechselwirkungen, die eine wichtige Rolle in Signaltransduktionspr... Full description
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Eine Vielzahl von Protein-Protein-Wechselwirkungen, die eine wichtige Rolle in Signaltransduktionsprozessen spielen, wird über Wechselwirkungen mit relativ kurzen Peptidsequenzen vermittelt. Dabei können vor allem über posttranslational modifizierte Proteinsequenzen Bindungen ausgelöst bzw. unterbunden werden. In der vorliegenden Arbeit wurden differentielle Peptid-Protein-Interaktionsexperimente (Pulldown-Experimente) mit phosphorylierten und den korrespondierenden unphosphorylierten Peptiden durchgeführt, um phosphorylierungsspezifische Interaktionspartner des Adapterproteins ADAP - als Teil des T-Zellsignalwegs und der adaptiven Immunantwort - zu bestimmen. Die Identifizierung der phosphorylierungsspezifisch interagierenden Proteine mit den ADAP-Peptiden erfolgte über quantitative Massenspektrometrie unter Verwendung von zwei Markierungsverfahren mit stabilen Isotopen: der Markierung von einzelnen Aminosäuren in Zellkultur (stable isotope labeling by amino acids in cell culture [SILAC]) und der enzymatischen 18O-Markierung mit schwerem Wasser.

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About the book

Full name Proteomische Methoden
Author Sabine Lange
Language German
Binding Book - Paperback
Date of issue 2011
Number of pages 144
EAN 9783838126845
ISBN 383812684X
Libristo code 06993712
Weight 222
Dimensions 152 x 229 x 10
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