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Nuclear Reprogramming

Methods and Protocols

Nathalie Beaujean

Hél?ne Jammes

Alice Jouneau

Language

English

Book Paperback

Libristo code: 13786406

Publishers Humana Press Inc., August 2016

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to pe... Full description

Libristo code: 13786406

292 b

42 931 Ft

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Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.