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Fed-Batch Fermentation

Language EnglishEnglish
Book Hardback
Book Fed-Batch Fermentation Garner G. Moulton
Libristo code: 05027888
Publishers Elsevier Books, January 2013
Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli... Full description
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Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli using a fed-batch fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - fed-batch fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

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About the book

Full name Fed-Batch Fermentation
Author Garner G. Moulton
Language English
Binding Book - Hardback
Date of issue 2013
Number of pages 188
EAN 9781907568923
ISBN 1907568921
Libristo code 05027888
Publishers Elsevier Books
Weight 442
Dimensions 237 x 164 x 16

Categories

Technology, Engineering, Agriculture > Biochemical engineering > Biotechnology
Medicine & Nursing > Pre-clinical medicine: basic sciences
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