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Enzyme Engineering

Name: Enzyme Engineering
Brand: Humana Press Inc.
SKU: 01433015
Price: 58286 HUF
Availability: InStock
Author: James Samuelson
ISBN: 9781627032926

Methods and Protocols

James Samuelson

Language

English

Book Hardback

Libristo code: 01433015

Publishers Humana Press Inc., February 2013

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological cat... Full description

Libristo code: 01433015

398 b

58 286 Ft

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Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.§Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.