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Developing Library-Based Bacterial Source Tracking Methods

Language EnglishEnglish
Book Paperback
Book Developing Library-Based Bacterial Source Tracking Methods Wail Hassan
Libristo code: 07173572
Publishers Scholars' Press, June 2014
The goal of this study is to develop efficient bacterial source tracking capabilities for the identi... Full description
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The goal of this study is to develop efficient bacterial source tracking capabilities for the identification of sources of fecal pollution. Two polymerase chain reaction-based methods, BOX-PCR and REP-PCR, were compared to pulsed field gel electrophoresis. Additionally, source tracking fidelity was compared using two target organisms, Escherichia coli and Enterococcus spp. The highest fidelity of source tracking was achieved using BOX-PCR and Enterococcus spp. The study also showed that applying a similarity value (a measure of how similar an unknown isolate is to isolates from a known source) threshold and/or quality factor (a measure of how an unknown isolate fits within the source to which it was assigned) threshold improved the fidelity of source tracking. Finally, enterococcal isolates obtained from local rivers and from Mississippi Gulf Coast were assigned to animal sources using the method developed as part of this work. The main source of fecal contamination in river samples from the Hattiesburg area appeared to be chicken feces, while most enterococcal isolates from the Gulf Coast samples were of gull or chicken origin, with occasional human isolates.

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About the book

Full name Developing Library-Based Bacterial Source Tracking Methods
Author Wail Hassan
Language English
Binding Book - Paperback
Date of issue 2014
Number of pages 120
EAN 9783639708745
ISBN 3639708741
Libristo code 07173572
Publishers Scholars' Press
Weight 186
Dimensions 152 x 229 x 7
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