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Bacteriophages

Methods and Protocols, Volume 3

Martha R. J. Clokie

Andrew M. Kropinski

Rob Lavigne

Language

English

Book Hardback

Libristo code: 16526778

Publishers Humana Press Inc., November 2017

This book expands on the previous edition with new chapters exploring emerging themes and methodolog... Full description

Libristo code: 16526778

318 b

47 781 Ft

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This book expands on the previous edition with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume I, Second Edition is a valuable resource for both established and novice phage scientists.