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Antibody Engineering

Name: Antibody Engineering
Brand: IntechOpen
SKU: 24122001
Price: 54631 HUF
Availability: InStock
Author: Thomas Böldicke
ISBN: 9789535138259

Thomas Böldicke

Language

English

Book Hardback

Libristo code: 24122001

Publishers IntechOpen, February 2018

Antibody Engineering comprises in vitro selection and modification of human antibodies including hum... Full description

Libristo code: 24122001

365 b

54 631 Ft

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Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.